![]() ![]() All entries were double-checked by one of the authors. Known samples were run as control on each day of use. Cells used for reverse grouping were pooled from three different known donor samples. Besides checking for appearance, test for specificity and pH of normal saline was carried out on each day of use. All reagents were subjected to quality control test including titer and avidity for each new lot used. Mitra prepared from different antibody producing human B-lymphocyte cell lines. Anti-D1 and anti D2 were anti-D reagents from two different manufacturers, i.e., Ortho Clinical Diagnostics and J. ![]() All anti-sera used were monoclonal reagents (the ABO monoclonal reagents are in vitro culture supernatants of hybridized immunoglobulin secreting mouse cell-lines) with a minimum titer of 1:256. Mitra and A cells, B cells and O cells prepared in house. The reagents used were anti-A, anti-B, anti-AB, anti D1, anti D2 manufactured by J. The grouping was carried out on an automated blood grouping machine (MITIS II, Ortho Clinical Diagnostics, Rochester, US). Present study seeks to provide data on ABO and Rh-D blood group distribution across India by compiling data from five regions of the country-North, South, East, West and Center.ĪBO and Rh grouping was performed for each sample on micro plates using standard procedure described in Dacie and Lewis. It is therefore important to have information on the distribution of these blood groups in any population.Īlthough, many studies have been conducted in various parts of India to determine the local ABO and Rh distribution, no pan-Indian study has so far been conducted to determine the country wide ABO and Rh distribution. The knowledge of the distribution of ABO and Rh blood groups is also essential for effective management of blood banks inventory. From the point of view of transfusion, rhesus blood group system is the second most important blood group.Īpart from their importance in blood transfusion practice, ABO and Rh blood groups are useful in population genetic studies, researching population migration patterns as well as resolving certain medico-legal issues, particularly disputed parentage. These antibodies are capable of producing hemolysis in vivo. The ABO system derives its importance from the fact that A and B are strongly antigenic and anti A and anti B occur naturally in the plasma of persons lacking the corresponding antigen. The ABO and Rh blood group system is the most important system in transfusion and organ transplants. ![]()
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